WebBy utilizing image cytometry, fluorescently labeled autophagosomes can be detected and measured via fluorescence analysis. ... (Cyto-ID® Green dye from Enzo Life Sciences) and GFP-LC3 protein in live cells. The image cytometry method has been shown to generate comparable fluorescence intensity data to flow cytometry, which can be an ... WebTo complement these tools, we also offer anti-GFP antibodies and antibody conjugates for a wide variety of applications, including imaging, western blotting, and flow cytometry. All of our anti-GFP antibodies are suited for detection of native GFP, GFP variants, and …
Resolving GFP and YFP on a Fortessa using unmixing Cytometry …
WebFeb 19, 2024 · Figure 1 Acquisition of phagocytosis assay by flow cytometry. Acquisition of neutrophil phagocytosis of S. aureus KV27 opsonized with normal human serum (NHS) at a 10:1 bacteria-to-cell ratio after 15 min incubation.(A) Gating of PMNs in the linear FSC and SSC, required to eliminate cell debris and possible free or clumped bacteria from the … WebFlow cytometry data analysis and figure preparation was done using FlowJo software. For the measurement of GFP signals in Fig. 3B, flow cytometry traces were fitted with two Gaussian functions (the first Gaussian corresponding to background fluorescence, the second Gaussian to specific GFP fluorescence). GFP signal is measured by the … how diagnose myocarditis
Flow cytometry of fluorescent proteins - ScienceDirect
WebApr 12, 2024 · Flow cytometry data were analyzed using FlowJo v.10.8.1 (Tree Star, Inc.). ... Reporter gene activation and T-cell activation were analyzed by measuring the MFI of GFP and CD69. The flow cytometry ... WebBackground: Mitochondrial protein import is typically measured by adding radiolabeled precursor proteins to isolated mitochondria. We have developed a novel, high-throughput method for measuring protein import in live differentiated PC12 cells using a tetracycline (Tet) regulated, nuclear encoded, mitochondrially-targeted GFP fusion protein and flow … WebAug 4, 2024 · M. smegmatis expressing the sigE-GFP construct was grown for different periods of time (0, 18, 48 h) as indicated and the expression of GFP was monitored by … how dial ireland